Effect of L-Arginine on the Thickness Iliac Arteries Wall Post Fogarty Balloon Embolectomy Catheter in Rabbit (Oryctolagus cuniculus)


The effect of the chronic administration of L-arginine on iliac arteries thickness after surgical embolectomy using Fogarty balloon catheter were examined. Sixteen New Zealand White male rabbits underwent unilateral iliac artery surgical embolectomy using Fogarty balloon catheter. Then the animals were divided into two group of control (P0) fed with standard rabbit diet and treatment (P1) standard rabbit diet added with L-arginine in their drinking water 4 for weeks. The iliac arteries were harvested for histological slide preparation with routine method, observed under the microscope attached with Nikon camera and measured using NIS-Element BR application in 8 zones. Data were collected and analyzed by independent T-test. The result of the vessel thickness was significantly decreased on the P1 (234.1± 18.1 µm) than P0 (411.6 ± 191.7 µm). Furthermore, the thickness of intimal was significantly decrease in P1 (140.9± 27.0 µm) compared with P0 (304.3± 215.7 µm). As a result, there was no significant different on the thickness of medial between P1 (93.2± 15.7 µm) and P0 (107.3± 101.5 µm). The results demonstrate that L-arginine decreased iliac artery wall thickness after surgical embolectomy with Fogarty balloon catheter in rabbit.


Keywords: L-arginine ; nitric oxide ; Fogarty balloon catheter ; vascular smooth muscle cell proliferation.

[1] D. Zhang, W. Lou, G. Chen and X. Yin, Endovascular Treatment Options of Acute Limb Ischemia, Int. J. Med. Phys. 2 (2013) 39-43.

[2] T. Bunt, Latrogenic Vascular Injury: A Discourse on Surgical Technique, New York, Futura Publishing Company, Inc. 20-29; 101-121, 1990.

[3] R. Rutherford, Arterial Diseases. In C. Zarins, & S. Glagov, Artery Wall Pathology in Atherosclerosis, Michigan, W.B. Saunders, 313-330, 1995.

[4] F. Doornekamp, C.Borst, C. Haudenschild and M. Post, Fogarty and Percutaneous Transluminal Coronary Angioplasty Balloon Injury Induce Comparable Damage to The Arterial Wall But Lead to Different Healing Responses. J. Vasc. Surg. 24 (196) 843-850.

[5] J. Chlupac, E. Filova and L. Acakova, Blood Vessel Replacement: 50 years of Development and Tissue, Physiol. Res. 58 (Suppl. 2) (2009) S119-S139.

[6] Y. Chen, J. Y. Jiang, H. B. Miao, X. J. Chen, X. J. Sun, and Y. J. Li, Hydrogen-rich Saline Attenuates Vascular Smooth Muscle Cellproliferation and Neointimal Hyperplasia by Inhibiting Reactive Oxygen Species

Production and Inactivating The Ras-ERK1/2-MEK1/2 and Akt Pathways, Int. J. Mol. Med. 31 (2013) 597-606.
[7] A.C. Newby and A.B. Zaltsman, Molecular Mechanisms in Intimal Hyperplasia, J. Pathol. 190.3. (2000) 300-309.

[8] M. Haga, A. Yamashita, J. Paszkowiak, B. Sumpio, and

A. Dardik, Oscillatory shear stress increases smooth muscle cell proliferation and akt phosphorylation, J. Vasc. Surg. 37.6. (2003) 1277–1284.

[9] G. Burnstock, Purinergic Signaling and Vascular Cell Proliferation and Death, Arterioscler. Thromb. Vasc. Biol. 22 (2002) 364-373.

[10] R.H. Boger, The Pharmacodynamics of L-Arginine, Amino Acid Nutrition Assessment Workshop, J. Nutr. 137 (2007) 1650S-1655S

[11] I.B.J.G. Debats, T.G.A.M. Wolfs, T. Gotoh, J.P.M. Cleutjens, C.J. Peutz-Kootstra, R.R.W.J. van der Hulst, Role of Arginine in Superficial Wound Healing In Man,
J. Niox. 21 (2009) 175-183.

[12] C.C. Huang, Chi, T.J. Lin, Y.F. Lu, C.C. Chen, C.Y. Huang and W.T. Lin, Protective Effects of L-Arginine Supplementation Against Exhaustive Exercise-Induced Oxidative Stress in Young Rat Tissues, Chin. J. Physiol. 52.5. (2009) 306-315.

[13] L. Jerca, O. Jerca, G. Mancaş, I. Constantinescu, R. Lupuşoru, Mechanism Of Action And Biochemical Effects Of Nitric Oxide (NO•), J. Preven. Med. 10.2. (2002) 35-45.

[14] J. Lei, Y. Vodovotz, E. Tzeng, T.R. Billiar, Nitric oxide, a protective molecule in the cardiovascular system. A review, J. Niox. 35 (2013) 175–185.

[15] C. Napoli, G. Paolisso, A. Casamassimi, M. Al-Omran,

M. Barbieri, L. Sommese, T. Infante, L.J. Ingnarro, Effects of Nitric Oxide on Cell Proliferation, J. Am. Coll. Cardiol. 62.2. (2013) 89-95.

[16] C.C.L. Wang, I. Gurevich, B. Draznin. Insulin Affects Vascular Smooth Muscle Cell Phenotype and Migration via Distinct Signaling Pathways, J. Diabetes. 10 (2003) 2562-2569.

[17] W.C. Tarry, R.G. Makhoul, L-Arginine Improves Endothelium-Dependent Vasorelaxation and Reduce Intimal Hyperplasia after Balloon Angioplasty, Arterioscler. Thromb. 14 (1994) 938-943.

[18] D.R. Janero, J.F. Ewing, Nitric Oxide and Postangioplasty Restenosis: Pathological Correlates and Therapeutic Potential, J. Free. Rad. Biomed. 29.12. (2000) 1199–1221.

[19] C.G. Pearce, S. F. Najjar, M. R. Kapadia, J. Murar, Beneficial Effect of A Short-Acting NO Donor for the Prevention of Neointimal Hyperplasia, J. Free. Rad. Biomed. 44 (2008) 73–81.

[20] J.A. Monlar, in Taylor & Francis Group (Ed.), Nutrition and Wound Healing. CRC Press, Boca Raton, 2007, Ch. 6.

[21] Y. Ariana, Pengaruh Pemberian Virgin Coconut Oil Terhadap Ketebalan Dinding Aorta Abdominalis Tikus Wistar Sesudah Diinduksi Aterogenesis, Thesis, Faculty and Cardiovascular Risk, J. Vasc. Bras. 13.2. (2014) of Medicine, Diponegoro University Semarang, 2006.

[22] S. Habib, A. Ali, Biochemistry of Nitric Oxide, Indian.

[26] W.K. Alderton, C. Cooper, R. Knowles, Nitric Oxide
J. Clin. Biochem. 26.1. (2011) 3–17. Synthases: structure, function, and inhibition, Biochem.

[23] J. Deanfield, J. Halcox, T. Rabelink, Endothelial J. 357 (2001) 593-615. Function and Dysfunction: Testing and Clinical

[27] V. Paul, P. Ekambaram, Involvement of Nitric Oxide in
Relevance, Circulation. 115 (2007) 1285-1295.
Learning & Memory Processes, Indian. J. Med. Res.

[24] R. Singh, S. Mengi, Y.J. Xu, A. Arneja, N. Dhalla,
133.5. (2011) 471–478.
Pathogenesis of Atherosclerosis: A Multifactorial

[28] L. Sorokin, The Impact of The Extracellular Matrix,
Process, Exp. Clin. Cardiol. 7.1. (2002) 40–53.
Nat. Rev. Immunol. 10.10. (2010) 712-723.

[25] B. Teixeira, A. Lopes, R. Macedo, C. Correa, T. Ramis,

[29] M. Yamakuchi, MicroRNAs in Vascular Biology, Int. J.
J. Ribeiro, Inflammatory Markers, Endothelial Function
Vasc. Med. (2012) 21-33.