Spectral Studies of Rat Bone Tissue in Modeling Osteoporosis and Effectiveness of Treatment By Hydroxyapatite
Abstract
Presents the result of experiments on the study of the model of osteoporosis in rats using Raman spectroscopy and the effectiveness of its treatment with hydroxyapatite. Were revealed spectral differences between groups of samples (control group, group with the model of osteoporosis and a group with the model of osteoporosis after treatment with hydroxyapatite). In addition, optical coefficients were introduced to evaluate the effectiveness of treatment.
Keywords: Raman spectroscopy, optical coefficients, osteoporosis, hydroxyapatite, collagen matrix
References
[1] Benevolenskaya L.I. The problem of osteoporosis in modern medicine // Scientific and practical rheumatology. - 2005. - № 1.
[2] McCreadie B. R. et al. Bone tissue compositional differences in women with and without osteoporotic fracture //Bone. – 2006. – Т. 39. – №. 6. – С. 1190-1195.
[3] William F. Finney, Effendi Widjaja, Steven A. Goldstein. Bone tissue compositional differences in women with and without osteoporotic fracture. Bone 39 (2006) 1190- 1195
[4] Orkoula M. G., Vardaki M. Z., Kontoyannis C. G. Study of bone matrix changes induced by osteoporosis in rat tibia using Raman spectroscopy //Vibrational Spectroscopy. – 2012. – Т. 63. – С. 404-408.
[5] A. Carden, R. M. Rajachar, M. D. Morris, D. H. Kohn. Ultrastructural Changes Accompanying the Mechanical Deformation of Bone Tissue: A Raman Imaging Study. Calcif Tissue Int (2003) 72:166–175
[6] Utzinger U. R. S. et al. Near-infrared Raman spectroscopy for in vivo detection of cervical precancers //Applied spectroscopy. – 2001. – Т. 55. – №. 8. – С. 955-959.
[7] Krafft C. et al. Disease recognition by infrared and Raman spectroscopy //Journal of biophotonics. – 2009. – Т. 2. – №. 1-2. – С. 13-28.
[8] Van de Poll S. W. E. et al. Raman spectroscopic investigation of atorvastatin, amlodipine, and both on atherosclerotic plaque development in APOE* 3 Leiden transgenic mice //Atherosclerosis. – 2002. – Т. 164. – №. 1. – С. 65-71.
[9] Takasaki E. Carbonate in Struvite Stone Detected in Raman Spectra Compared with Infrared Spectra and X-Ray Diffraction //International journal of urology. – 1996. – Т. 3. – №. 1. – С. 27-30.
[10] Schut T. C. B. et al. Discriminating basal cell carcinoma from its surrounding tissue by Raman spectroscopy //Journal of Investigative Dermatology. – 2002. – Т. 119. – №. 1. – С. 64-69.
[11] Matousek P. Deep non-invasive Raman spectroscopy of living tissue and powders //Chemical Society Reviews. – 2007. – Т. 36. – №. 8. – С. 1292-1304.
[12] Morris M. D., Mandair G. S. Raman assessment of bone quality //Clinical Orthopaedics and Related Research®. – 2011. – Т. 469. – №. 8. – С. 2160-2169.
[13] Minaeva S.A., et al., ”The use of Raman spectroscopy for studying the mineralization of bone regenerates,” // Clinical and Experimental Morphology. – 2012. – Vol. 4. – pp. 53-56.
[14] Botelho M. A. et al. Nanostructured transdermal hormone replacement therapy for relieving menopausal symptoms: a confocal Raman spectroscopy study //Clinics. – 2014. – Т. 69. – №. 2. – С. 75-82.
[15] Timchenko, E.V., Timchenko, P.E.,Volova, L.T., Ponomareva, Yu.V.,Taskina, L.A. Raman spectroscopy of the organic and mineral structure of bone grafts // Quantum Electronics, 2014 - 44 (7) – P. 696 – 699.
[16] Timchenko P. E., Timchenko E. V., Pisareva E. V., Vlasov M. Yu., Red’kin N. A., Frolov O. O. Spectral analysis of allogeneic hydroxyapatite powders // IOP Conf. Series: Journal of Physics: Conf. Series 784 (2017) 012060 doi:10.1088/1742 6596/784/1/012060.
[17] Timchenko E.V., Tregub N.V., Taskina L.A., Selezneva E. A., Timchenko P.E. Optical methods for control of hydrogen influence on plants // Proc. of SPIE Vol. 9221, Remote Sensing and Modeling of Ecosystems for Sustainability XI, pp. 922108 (2014) doi:10.1117/12.2061357.
[18] Timchenko E.V., Timchenko P.E., Volova L.T., Pershutkina S.V., Shalkovsky P.Y. OPTICAL ANALYSIS OF AORTIC IMPLANTS, Optical Memory and Neural Networks, 2016, V.25, №3, pp.192-197.
[19] Morris M.D., Carden A., Rajachar R.M., Kohn D.H. (2001) Bone microstructure deformation observed by Raman spectroscopy. Proc SPIE 4254:81–89.
[20] Polak R., Pitombo R. N. M. Care during freeze-drying of bovine pericardium tissue to be used as a biomaterial: a comparative study //Cryobiology. – 2011. – Т. 63. – №. 2. – С. 61-66.
[21] Timchenko E.V., Timchenko P.E., Taskina L.A., Volova L.T., Miljakova M.N.; Maksimenko N.A. Using Raman spectroscopy to estimate the demineralization of bone transplants during preparation // JOURNAL OF OPTICAL TECHNOLOGY,2015 – V. 82 – №3. Pp.153-157.