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TimchenkoP. Е., TimchenkoЕ. V., VolovaL. Т., FrolovО. О., & Kiyko, N. K. (2018). Raman Spectroscopy for Analysis of Implants from the Dura Mater. KnE Energy, 3(2), 500–506. https://doi.org/10.18502/ken.v3i2.1857

https://doi.org/10.18502/ken.v3i2.1857

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authors

  • P Е Timchenko
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  • Е V Timchenko
    No author data available.
  • L Т Volova
    No author data available.
  • О О Frolov
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  • N K Kiyko
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Published Date

  • Apr 17, 2018

Raman Spectroscopy for Analysis of Implants from the Dura Mater

KnE Energy / The 2nd International Symposium "Physics, Engineering and Technologies for Biomedicine" / Pages 500–506

Abstract

In this paper we present results of the comparative evaluation of the structural properties of the dura mater specimens (DM), manufactured using the ”Lioplast” technology, used in the clinic in the field of atrophic processes in multiple gums recessions, using the Raman spectroscopy (RS) method. The introduced coefficients and a two-dimensional analysis that showed that the processing retains the main components and removes DNA / RNA, which increases the quality that provides access to quality materials in the treatment of multiple gum recessions. It was found that the main differences appear at wavenumbers of 835 cm−1 (tyrosine), 855 cm−1 (proline), 940 and 1167 cm−1 (GAGs, CSPGs), 1240 cm−1 (amide III), 1560 cm−1 (amide II) and 1447 cm−1 (lipids and proteins). It is shown that Raman spectroscopy can be used to evaluate implants from the dura mater.


Keywords: Raman spectroscopy, dura mater, biomaterial, spectral analysis.

References
[1] Muslimov S А 2000 Morphological Aspects of Regenerative Surgery (Ufa, Bashkortostan) 168 p. (in Russian)


[2] Ganja I R 2007 Recession of the gums. Diagnosis and treatment methods: a manual for physicians (Samara: Sodruzhestvo) 84 p. (in Russian)


[3] Ferraro J R, Nakamoto K 1994 Introductory Raman Spectroscopy (Academic Press, San Diego).


[4] Chen H, Xu P W, Broderick N 2016 In vivo spinal nerve sensing in MISS using Raman spectroscopy (In Proceedings of SPIE Vol. 9802 (pp. 98021L). Las Vegas: Society of Photo-optical Instrumentation Engineers (SPIE)). doi:10.1117/12.2218783


[5] Chen J L, Duan L, Zhu W 2014 ( J Transl Med 12: 88.) https://doi.org/10.1186/1479- 5876-12-88


[6] Saxena T, Deng B, Stelzner D, Hasenwinkel J, Chaiken J. 2011 Raman spectroscopic investigation of spinal cord injury in a rat model ( Journal of Biomedical Optics, 16 (2)), art. no. 027003.


[7] Timchenko P E, Zakharov V P, Volova L T, Boltovskay V V, Timchenko E V Diagnostics of bone implantat and control of their process osteointegration with of a method confocal microscopy (Computer Optics 35 (2)), pp. 183-1872011.


[8] Timchenko E V, Timchenko P E, Taskina L A, Volova L T, Miljakova M N, Maksimenko N A 2015 Using Raman spectroscopy to estimate the demineralization of bone transplants during preparation ( Journal of optical technology – V. 82 – №3), Pp. 153- 157.


[9] Thomas G J, 1976 Raman spectroscopy of viruses and protein-nucleic acid interactions (The SPEX Speacker Industries Inc. Vol. XXI No.4).


[10] David I E, David P, Cowcher L A, O’Hagana S, Royston G 2013 Illuminating disease and enlightening biomedicine: Raman spectroscopy as a diagnostic tool (Analyst, 138, p. 3871. ISSN 0003-2654).


[11] Cristina M M, Halmagyi A, Mircea D 2009 Puiac and Ioana Pavel FT-Raman signatures of genomic DNA from plant tissues (Spectroscopy 23 59–70 DOI 10.3233/SPE-2009- 0375).


[12] Benevides J M, Overman S A, Thomas G J, 2005 ( J. Raman Spectrosc. 36, 279–299).


[13] Ruiz-Chica A J, Medina M A, Sanchez-Jimenez F, Ramirez F J 2004 Characterization by Raman spectroscopy of conformational changes on guaninecytosine and adeninethymine oligonucleotides induced by aminooxy analogues of spermidine ( Journal of Raman Spectroscopy, 35: 93–100).