The Size of Vesicles Produced By Different Stem Cells

Abstract

Extracellular vesicles (EVs) are now intensively studied in the context of their action on the irradiated organism. Suspensions of EVs isolated from culture media of two different cell lines were analyzed by transmission electron microscopy and nanoparticle tracking analysis (NTA) to evaluate size and concentration of contained particles. The results obtained indicate the difference in analyzed parameters depending on the cell type.


Keywords: stem cells, extracellular vesicles, nanoparticle tracking analysis

References
[1] M.V. Vyalkina, I.B. Alchinova, E.N. Yakovenko, Yu.S. Medvedeva, I.N. Saburina, and M.Yu. Karganov, “Long-Term Effects of Stem Cells on Total-Body Irradiated Mice”, IOP Conf. Series: Journal of Physics: Conf. Series, vol.784, pp. 012015, 2017, doi:10.1088/1742-6596/784/1/012015


[2] J.S. Schoefinius, B. Brunswig-Spickenheier, T. Speiseder, S. Krebs, U. Just, and C. Lange, “Mesenchymal Stromal Cell-Derived Extracellular Vesicles Provide Longterm Survival after Total Body Irradiation without additional Hematopoietic Stem Cell Support”, Stem Cells, doi:10.1002/stem.2716


[3] S. Wen, M. Dooner, Y. Cheng, E. Papa, M. Del Tatto, M. Pereira,..., and C. Stewart, “Mesenchymal stromal cell derived extracellular vesicles rescue radiation damage to murine marrow hematopoietic cells”, Leukemia, vol.30(11), pp. 2221-2231, 2016, doi:10.1038/leu.2016.107


[4] P.A. Zuk, M. Zhu, H. Mizuno, J. Huang, J.W. Futrell, A.J. Katz, P. Benhaim, H.P. Lorenz, and M.H. Hedrick, “Multilineage cells from human adipose tissue: implications for cell-based therapies”, Tissue engineering, vol.7(2), pp. 211-228, 2004, doi.:10.1089/107632701300062859


[5] C. Lässer, M. Eldh, and J. Lötvall, ”Isolation and characterization of RNAcontaining exosomes”, Journal of visualized experiments: JoVE, №59, e3037, 2012, doi:10.3791/3037

[6] O.N. Shustova, O.A. Antonova, N.V. Golubeva, S.G. Khaspekova, V.V. Ya-kushkin, S.A. Aksuk, I.B. Alchinova, M.Y. Karganov, and A.V. Mazurov, “Differential procoagulant activity of microparticles derived from monocytes, granulocytes, platelets and endothelial cells: impact of active tissue factor”, Blood Coagulation & Fibrinolysis, vol.28, №5, pp. 373-382, 2017, doi:10.1097/MBC.0000000000000609